[Top Left] Using non-negative matrix factorisation (NMF), we can identify key dynamic features across whole-brain calcium fluorescence. These show distinct temporal signatures that show significant group level differences between gabra1 -/-, gabrg2 -/-, and wildtypes larvae.

[Bottom Right] NMF factorises the time-varying calcium signal into time-invariant factors (shown here), and their associated time-varying expression (top panels)

[Top Right] Plotting three dimensions from the NMF against each other, we can visualise the spread of whole-brain activity in a reduced state space. This shows distinct divergence of the different genotype [Dynamic content: Add from top-left panel]. The maximal separation occurs early in the experiments, immediately following light exposure [Dynamic content: Hovering over state space will indicate corresponding time points in the top left plot]